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Now before the electricity flows to detect light, another compound is introduced in a known quantity. in this case, it is more estradiol that has already been “biotinylated” (estradiol with a vitamin b7 attached to it). Biotin and streptavidin are extremely attracted to each other, so this new estradiol (that will not emit light) competes with the existing estradiol to bind to the magnetic beads. The idea is that the more estradiol that was already in the blood, the more of this new labeled estradiol gets competed off and because this alters the total light emission, you can extrapolate how much estradiol was in the sample originally.